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superdex 200 increase gel filtration column  (Cytiva Europe)


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    Structured Review

    Cytiva Europe superdex 200 increase gel filtration column
    Superdex 200 Increase Gel Filtration Column, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 4852 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superdex+200+increase+gel+filtration+column/Superdex+200+Increase+SEC+column/us12655220-1416-24-33
    Average 99 stars, based on 4852 article reviews
    superdex 200 increase gel filtration column - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Purification:

    Article Title: Ligand-binding fusion proteins
    Article Snippet: Purification of IL-2 N88D without antibody molecule was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 75 increase gel filtration column (Cat. No: 29148721, GE Healthcare). .. Purification of IL-2 fused antibodies was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 200 increase gel filtration column (Cat. No: 28990944, GE Healthcare). ..

    Article Title: The RPA-binding domain and the KKRK motif in Rad26ATRIP cooperate at the perturbed DNA replication fork for initiating checkpoint signalling.
    Article Snippet: The flow-through was then passed through a heparin column (Cytiva) and eluted with a gradient of 0-1.0 M NaCl in a buffer of 20 mM HEPES, pH 7.5, 10% glycerol, and 0.3 mM TCEP. .. Fractions containing Ssb1-N were pooled and concentrated, then further purified on a Superdex 200 increase gel filtration column (Cytiva) in a buffer containing 20 mM HEPES, pH 7.5, 200 mM NaCl, 0.3 mM TCEP. ..

    Article Title: Design and structural characterization of autoinhibition-compromised full-length Ran
    Article Snippet: .. The proteins were purified on a Superdex 200 increase gel filtration column on Äkta Pure (GE Healthcare) using a buffer containing 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM MgCl2, 5 mM BME. .. Purified proteins were concentrated to 5-10 mg/mL using a Vivaspin turbo 15 (Sartorius) concentrator, and store at -80 ̊C.

    Article Title: STK19 is a DNA/RNA-binding protein critical for DNA damage repair and cell proliferation.
    Article Snippet: STK19 was originally identified as a manganese-dependent serine/threonine-specific protein kinase, but its function has been highly debated.. Here, the crystal structure of STK19 revealed that it does not contain a kinase domain, but three intimately packed winged helix (WH) domains.. The third WH domain mediated homodimerization and double-stranded DNA binding, both being important for its nuclear localization.

    Article Title: Structure-guided discovery of aminopeptidase ERAP1 variants capable of processing antigens with novel PC anchor specificities.
    Article Snippet: .. The entire purification process was performed at 4 C. The eluted proteins from Ni-NTA resin were concentrated to 4 mg/mL in 500 μL for further purification with a Superdex 200 Increase gel filtration column (Cytiva). ..

    Affinity Purification:

    Article Title: Ligand-binding fusion proteins
    Article Snippet: Purification of IL-2 N88D without antibody molecule was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 75 increase gel filtration column (Cat. No: 29148721, GE Healthcare). .. Purification of IL-2 fused antibodies was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 200 increase gel filtration column (Cat. No: 28990944, GE Healthcare). ..

    Size-exclusion Chromatography:

    Article Title: Ligand-binding fusion proteins
    Article Snippet: Purification of IL-2 N88D without antibody molecule was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 75 increase gel filtration column (Cat. No: 29148721, GE Healthcare). .. Purification of IL-2 fused antibodies was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 200 increase gel filtration column (Cat. No: 28990944, GE Healthcare). ..

    Article Title: STK19 is a DNA/RNA-binding protein critical for DNA damage repair and cell proliferation.
    Article Snippet: STK19 was originally identified as a manganese-dependent serine/threonine-specific protein kinase, but its function has been highly debated.. Here, the crystal structure of STK19 revealed that it does not contain a kinase domain, but three intimately packed winged helix (WH) domains.. The third WH domain mediated homodimerization and double-stranded DNA binding, both being important for its nuclear localization.

    Filtration:

    Article Title: Ligand-binding fusion proteins
    Article Snippet: Purification of IL-2 N88D without antibody molecule was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 75 increase gel filtration column (Cat. No: 29148721, GE Healthcare). .. Purification of IL-2 fused antibodies was done using affinity purification by HisTrap excel (Cat. No: 17371206, GE Healthcare) followed by size exclusion chromatography using Superdex 200 increase gel filtration column (Cat. No: 28990944, GE Healthcare). ..

    Article Title: PARP1 regulates the genomic ribonucleotide processing activity of TOP1 to prevent the formation of toxic TOP1-DNA adducts and the associated mutations
    Article Snippet: .. The eluted protein was finally loaded into a Superdex 200 increase gel filtration column (Cytiva) connected to an AKTA pure chromatography system (Cytiva) and eluted with K500 buffer. ..

    Article Title: The RPA-binding domain and the KKRK motif in Rad26ATRIP cooperate at the perturbed DNA replication fork for initiating checkpoint signalling.
    Article Snippet: The flow-through was then passed through a heparin column (Cytiva) and eluted with a gradient of 0-1.0 M NaCl in a buffer of 20 mM HEPES, pH 7.5, 10% glycerol, and 0.3 mM TCEP. .. Fractions containing Ssb1-N were pooled and concentrated, then further purified on a Superdex 200 increase gel filtration column (Cytiva) in a buffer containing 20 mM HEPES, pH 7.5, 200 mM NaCl, 0.3 mM TCEP. ..

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module.
    Article Snippet: VcParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21- bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein- DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..

    Article Title: Design and structural characterization of autoinhibition-compromised full-length Ran
    Article Snippet: .. The proteins were purified on a Superdex 200 increase gel filtration column on Äkta Pure (GE Healthcare) using a buffer containing 20 mM Tris pH 8.0, 100 mM NaCl, 5 mM MgCl2, 5 mM BME. .. Purified proteins were concentrated to 5-10 mg/mL using a Vivaspin turbo 15 (Sartorius) concentrator, and store at -80 ̊C.

    Article Title: STK19 is a DNA/RNA-binding protein critical for DNA damage repair and cell proliferation.
    Article Snippet: STK19 was originally identified as a manganese-dependent serine/threonine-specific protein kinase, but its function has been highly debated.. Here, the crystal structure of STK19 revealed that it does not contain a kinase domain, but three intimately packed winged helix (WH) domains.. The third WH domain mediated homodimerization and double-stranded DNA binding, both being important for its nuclear localization.

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module
    Article Snippet: Vc ParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21-bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein-DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..

    Article Title: Structure-guided discovery of aminopeptidase ERAP1 variants capable of processing antigens with novel PC anchor specificities.
    Article Snippet: .. The entire purification process was performed at 4 C. The eluted proteins from Ni-NTA resin were concentrated to 4 mg/mL in 500 μL for further purification with a Superdex 200 Increase gel filtration column (Cytiva). ..

    Chromatography:

    Article Title: PARP1 regulates the genomic ribonucleotide processing activity of TOP1 to prevent the formation of toxic TOP1-DNA adducts and the associated mutations
    Article Snippet: .. The eluted protein was finally loaded into a Superdex 200 increase gel filtration column (Cytiva) connected to an AKTA pure chromatography system (Cytiva) and eluted with K500 buffer. ..

    Incubation:

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module.
    Article Snippet: VcParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21- bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein- DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module
    Article Snippet: Vc ParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21-bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein-DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..

    Injection:

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module.
    Article Snippet: VcParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21- bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein- DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..

    Article Title: Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module
    Article Snippet: Vc ParD2:ParE2 (100 μM) (6:2) complex was incubated with 50 μM 33- or 21-bp DNA operator variants in 500 μl of final volume (sequences given in the table in fig. S7). .. Protein-DNA complexes were incubated at 25°C for 15 min before injection onto a Superdex 200 Increase gel filtration column (GE Healthcare). ..



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